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miRNA biogenesis:
An miRNA gene is transcribed, generally by RNA polymerase II (Pol II), generating the primary miRNA (pri-miRNA). In the nucleus, the RNase III endonuclease Drosha and the double-stranded RNA-binding domain (dsRBD) protein DGCR8/Pasha cleave the pri-miRNA to produce a 2-nt 3'overhang containing the ~70-nt precursor miRNA (pre-miRNA). Exportin-5 transports the pre-miRNA into the cytoplasm, where it is cleaved by another RNase III endonuclease, Dicer, together with the dsRBD protein TRBP/Loquacious, releasing the 2-nt 3'overhang containing a ~21-nt miRNA:miRNA* duplex. The miRNA strand is loaded into an Argonaute-containing RNA-induced silencing complex (RISC), whereas the miRNA* strand is typically degraded.
The vector-based miRNA technology includes: customer provides the miRNA sequences, reliable DNA oligo synthesis, custom miRNA plasmid construction, ready plasmid DNA preparation, plasmid DNA sequencing, deliver the products.
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