RISO is a fast and convenient RNA extraction reagents, from the cells, tissues and other materials extracted RNA. In the sample processing, RISO cracking can be completely full sample, while maintaining the integrity of RNA. Centrifugal join chloroform, a medium solution of water, the middle and lower classes of organic phase. For medium-and membership isopropanol precipitation recovery RNA; middle layer using ethanol precipitation can be recovered DNA; organic phase of recovery available isopropanol precipitation.

RISO reagent composition

Note: 2-8 ° C dark saved 12 months; transport can be at room temperature. Main features:

1. anti-degradation: RNA enzymes (RNase) in the RISO in rapid inactivation to ensure that the RNA extract high-quality, high-volume, repeatable.

2. cells, tissues are applicable: applied to various cells and specimens, a small amount of tissue (such as biopsy specimens) in particular, apply.

3. operation simple, time-saving, even if the initial operation will also apply.

RISO reagent can be used for a small number of samples (such as biopsy specimens) RNA extraction, can also be used for large sample (such as the tissues and organs) RNA extraction. RNA from the whole process to facilitate rapid, and the entire operation process can be completed within one hour, extract the DNA and RNA protein-free of pollution, Gao purity, can be used directly in Northern Blotting (RNA blot analysis), dot blot, further Poly (A) + (mRNA) purification, in vitro translation, RNase protection analysis, RT-PCR, Construction cDNA library, and other molecular biology experiments.