The Pfu DNA Polymerase is a highly thermostable DNA polymerase. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5'=>3' direction. The Pfu DNA Polymerase also exhibits 3'=>5' exonuclease (proofreading) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5'=>3' exonuclease activity. Consequently, Pfu DNA Polymerase is useful for polymerization reactions requiring high fidelity synthesis

Applications
High fidelity PCR.
Blunt-end PCR cloning.
Site-directed mutagenesis.

Concentration
5 U/ ul

Stability and Storage
The recommended storage condition is -20°C. Avoid RT and repeated freeze/thaws.

Specification (Units)
250U, 1000U

Unit Definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmole of dNTPs into acid insoluble material in 30 minutes at 75°C.

Reagents Supplied
10×PCR Buffer (Mg²⁺ Plus):
200mM Tris-HCl (pH8.8,25℃), 100mM KCl , 160mM (NH₄)₂SO₂ , 20mM MgSO₄ , 1.0% TritonX-100 , 1mg/ml BSA.

Quality Control
The absence of endodeoxyribonucleases and exodeoxyribonucleases confirmed by appropriate quality tests. Functionally tested in PCR. Purity tested by SDS-PAGE.